A high-density collection of EMS-induced mutations for TILLING in Landsberg erecta genetic background of Arabidopsis
نویسندگان
چکیده
منابع مشابه
High-throughput TILLING for Arabidopsis.
Targeting induced local lesions in genomes (TILLING) is a general strategy for identifying induced point mutations that can be applied to almost any organism. In this chapter, we describe the basic methodology for high-throughput TILLING. Gene segments are amplified using fluorescently tagged primers, and products are denatured and reannealed to form heteroduplexes between the mutated sequence ...
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doi: 10.1111/j.1399-3054.2006.00809.x Among the Arabidopsis thaliana accessions Columbia (Col), Landsberg erecta (Ler), and Wassilewskija (Ws), Ler and Ws showed higher copper (Cu) tolerance than Col, while accumulating more Cu. Thus, Cu tolerance did not appear to be related to metal exclusion. Rather, the higher Cu tolerance of Ler and Ws may reflect less Cu-induced nutrient deficiency as the...
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New Arabidopsis recombinant inbred lines (Landsberg erecta x Nossen) reveal natural variation in phytochrome-mediated responses.
We used 52 Arabidopsis (Arabidopsis thaliana) accessions and developed a new set of 137 recombinant inbred lines between Landsberg erecta (Ler) and Nossen (No-0) to explore the genetic basis of phytochrome-mediated responses during deetiolation. Unexpectedly, most accessions showed weak or moderate hypocotyl growth and cotyledon unfolding responses to pulses of far-red light (FR). Crosses betwe...
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ژورنال
عنوان ژورنال: BMC Plant Biology
سال: 2009
ISSN: 1471-2229
DOI: 10.1186/1471-2229-9-147